Fig. 6

Effects of shHSP70–3/−4 blockade on the protective effects of MANF against 6-OHDA induced apoptosis in SHSY-5Y cells. a, b SHSY-5Y cells were pretreated with control RNAi of shHSP70–3/−4 for 48 h and then stimulated with 50 μM 6-OHDA or 50 μM 6-OHDA + 4 μg/mL MANF for further 48 h. Cell viability was measured by TUNEL and MTT. c A representative Western blot result shows the expression of cleaved caspase-3 in control SHSY-5Y cells, SHSY-5Y cells treated with 150 μM 6-OHDA +4 μg/mL of MANF; 150 μM of 6-OHDA; A representative Western blot result shows the expression of cleaved caspase-3 in HSP70–3/−4RNAi SHSY-5Y cells treated with 150 μM 6-OHDA +4 μg/mL of MANF and 150 μM of 6-OHDA. (*p < 0.05; one-way ANOVA with Bonferroni post-tests)