Fig. 6

miRNA agomir treatment counteracts Aβ pathology in APP/PS1 mice. a The co-localization of Aβ plaques (stained by 6E10) and CPE in the hippocampus of 10–11-MO APP/PS1 mice. Blue dotted circles represent cored plaques while the white dotted circle indicates diffuse plaque. Scale bars, 100 μm (top panel), 20 μm (bottom panels). High magnifications of the two forms of Aβ plaques (cored and diffuse) are shown in the lower panels. b Various protein immunoreactivity of the cored CPE plaques in the hippocampus of 10–11-MO APP/PS1 mice. The leftmost panels show immunostaining for cored CPE plaque and aggregatin (yellow arrows) in adjacent sections (indicated by features marked with asterisks) of hippocampus in APP/PS1 mice. The white dotted circles indicate diffuse plaque. Scale bars, 100 μm (bottom left panel), 20 μm (other panels). c mBDNF immunoreactivity of the cored and diffuse CPE plaques in the hippocampus of 10–11-MO APP/PS1 mice. Scale bar, 20 μm. d Aβ and mBDNF immunoreactivity for the cored CPE plaques in the hippocampus of 10–11-MO APP/PS1 mice. Yellow circles indicate the growing of the CPE plaque core. Scale bar, 20 μm. e CPE plaques in the hippocampus of 10–11-MO APP/PS1 mice were associated with clusters of activated microglia (Iba1-positive). Scale bar, 20 μm. f Iba1-positive microglial cell bodies were located in close vicinity to the aggregatin-positive plaque core. Yellow arrows indicate microglial cell bodies. Scale bar, 10 μm. g Representative images and quantification showed that cored but not total CPE plaques (stained by CPE, indicated by yellow triangles) were reduced in the DG of 10–11-MO APP/PS1 mice 5 weeks after the completion of agomir treatment. Scale bar, 100 μm. Data are presented as mean ± SEM, n = four mice each group. h Fibrillar Aβ plaques (stained by ThS) was reduced 5 weeks after the completion of agomir treatment in 10–11-MO APP/PS1 mice. Representative images and quantification data from the hippocampus are shown. Scale bar, 100 μm. Data are presented as mean ± SEM, n = five mice each group. For (g) and (h), data were analyzed with one-way ANOVA