Fig. 5
Cav1.2 and Cav3.2 are differentially expressed in A53T striatal astrocytes. a–d Representative confocal images of striatal sections co-stained with antibodies against Cav1.2 (a), Cav3.1 (b), Cav3.2 (c), and Cav3.3 (d) and the astrocyte marker, GFAP. DAPI (blue) was used for nuclei staining. Images on the right represent magnification of the boxed area. Scale bar: 50 μm (10 μm for the magnification images). e–h Quantification of mean fluorescence intensity of Cav VGCCs in individual GFAP+ astrocytes following 3D reconstruction in three independent pairs (1, 2, 3) of WT and A53T Tg mice; (e) for Cav1.2, #P < 0.0001, n ≥ 68 astrocytes for each genotype, f for Cav3.1, P = 0.3298, 0.1878, 0.0950 for pairs 1, 2, 3 respectively and n ≥ 44 astrocytes for each genotype, g for Cav3.2, #P < 0.0001, n ≥ 129 astrocytes, and h for Cav3.3, P = 0.2434, 0.2479, 0.0955, n ≥ 16 for each genotype. i Pre-averaged values of Cav mean fluorescence intensity relative to WT for 3 WT and A53T animal pairs. Data are presented as means ± SEM. All statistics by unpaired Student’s t test