Fig. 1

CARS protein level in the temporal cortex increases with aging and in AD. a Correlation between the CARS-ir intensity in the temporal cortex and age of the control subjects. b Representative images of CARS staining in the post-mortem temporal cortex of subjects with different Braak stages. Insets: Magnifications of the white dotted boxes. c Quantification of the CARS-ir intensity in panel b. d and e Representative images of western blotting (d) and quantification (e) of CARS protein level in the post-mortem temporal cortex of subjects with different Braak stages. f Representative images of neuronal CARS expression in the post-mortem temporal cortex of subjects with different Braak stages. White arrowheads denote the co-localization of CARS-ir and NeuN. g Quantification of the density of neurons. h Quantification of CARS-ir intensity per neuron in the temporal cortex of subjects with different Braak stages. Data are shown as mean ± SEM; *P < 0.05, ***P < 0.001, ****P < 0.0001