Fig. 7
NPs mediate fAβ-induced NF-ĸB nuclear translocation in microglia. a, b BV2 microglia were treated with 20 µM fAβ with or without NPs for 2 h. Cells were immediately fixed with 4% PFA. Cells were then blocked using 2% blocking buffer, incubated with anti-NF-ĸB P65 antibody overnight at 4 °C, and washed with PBS-T. Cells were incubated with secondary antibody Alex 488 for 1 h and nuclei were counterstained with Hoechst (blue). a Representative confocal microscopy images showing NF-ĸB nuclear translocation. Scale bar, 25 µm. b Quantitative measurement of the nuclear translocation of NF-ĸB in BV2 cells treated with Aβ with or without NPs for 2 h. Data are presented as mean ± SEM; n = 3; **P = 0.0027 for fAβ versus control, **P = 0.0044 for T12P5 versus fAβ and **P = 0.0054 for M12P5 versus fAβ, for Dunnett’s multiple comparisons shown on graph by one-way ANOVA