Fig. 5
Effect of NPs on amyloid beta (Aβ) cellular uptake. BV2 microglia were treated with NPs for 24 h and then co-incubated with Alexa fluor 488-labeled fAβ for 24 h. Cells were fixed with 4% PFA and then washed two times with PBS to remove extracellular fAβ. Cells were then incubated with 0.5% Triton-X-100 in potassium buffered saline (PBS-T) to remove any membrane-bound fAβ particles. a Confocal images of intracellular Alexa fluor 488-labeled fAβ (green) and brightfield illuminated cells. Scale bar, 50 µm. b Quantitative measurement of the intracellular fluorescence of Aβ488 in BV2 microglia. Data are presented as mean ± SEM; n = 4; **P = 0.0016 for T12P5 (PS) versus fAβ488, and **P = 0.0059 for M12P5 (PS) versus fAβ488, for Dunnett’s multiple comparisons shown on graph by one-way ANOVA. c Thioflavin-S stain (blue) of fAβ in BV2 microglia (brightfield/grey) treated with NPs for 24 h, and then co-incubated with fAβ for 24 h. d Quantitative measurement of the intracellular fluorescence of Thioflavin-S stain in BV2 microglia. Data are presented as mean ± SEM; n = 4; ****P < 0.0001 for T12P5(PS) versus fAβ488, and ****P < 0.0001 for M12P5(PS) versus fAβ488, for Dunnett’s multiple comparisons shown on graph by one-way ANOVA