Fig. 4

Scavenger receptors interrupt the microglial internalization of fibril amyloid beta (fAβ). BV2 microglia were treated with antibodies against scavenger receptors CD36, CD68, TLR2, and SRA1 or isotype control for 24 h, and then co-incubated with Alexa fluor 488-labeled fAβ for 24 h. The cells were fixed with 4% PFA, then washed two times with PBS to remove the extracellular fAβ. Cells were then incubated with 0.5% Triton-X-100 in potassium buffered saline (PBS-T) to remove any membrane-bound fAβ particles. a Representative confocal images of intracellular fAβ488 (green) and brightfield illuminated cells (grey). Scale bar, 50 µm. b Quantitative measurement of the intracellular Aβ488 in BV2 cells. Data are presented as mean ± SEM; n = 4; ****P < 0.0001 for Dunnett’s multiple comparisons shown on graph by one-way ANOVA