Fig. 5

Sex-specific changes in the levels of VEGF receptors in the 5×FAD mouse brain. (a) Representative images and (b) quantitative analysis of full-length VEGFR1 (VEGFR1^FL) and soluble VEGFR1 (sVEGFR1) protein levels in the hippocampus of female 5×FAD mice (n = 7–10 mice per group). (c) Representative images and (d) quantitative analysis of VEGFR1^FL and sVEGFR1 protein levels in the cortex of female 5×FAD mice (n = 7–10 mice per group). (e) Representative images and (f) quantitative analysis of VEGFR2 protein levels in the hippocampus of female 5×FAD mice (n = 7–9 mice per group). (g) Representative images and (h) quantitative analysis of VEGFR2 protein levels in the cortex of female 5×FAD mice (n = 7–9 mice per group). (i) Representative images and (j) quantitative analysis of VEGFR1^FL and sVEGFR1 protein levels in the hippocampus of male 5×FAD mice (n = 4–5 mice per group). (k) Representative images and (l) quantitative analysis of VEGFR1^FL and sVEGFR1 protein levels in the cortex of male 5×FAD mice (n = 4–5 mice per group). (m) Representative images and (n) quantitative analysis of VEGFR2 protein levels in the hippocampus of male 5×FAD mice (n = 8–10 mice per group). (o) Representative images and (p) quantitative analysis of VEGFR2 protein levels in the cortex of male 5×FAD mice (n = 8–10 mice per group). All data are presented as the mean ± SEM and were analyzed by one-way ANOVA followed by Fisher’s LSD test. WT (Veh): wild-type littermates receiving sham treatment, AD (Veh): 5×FAD mice receiving sham treatment, AD (Bev): 5×FAD mice receiving bevacizumab treatment