Fig. 8

Metabolomics analysis of iPSC-derived CNSC-SE. a HCA was performed on peaks, and the distances between peaks are represented in tree diagrams. b PC1 and PC2 show the first principal component and second principal component respectively. The numbers in parentheses represent the contribution rate. c Comparative analysis of Human Metabolome Database physical property classification between CNSC-SE and iPSC-SE. d Venn diagram of total metabolomics compound from four batches. Data are presented as mean ± SEM. Welch’s T-test; *P < 0.05, **P < 0.01, ***P < 0.001. iPSC, induced pluripotent stem cells; CNSC-SE, cortical neural stem cell secretome; GFAP, glial fibrillary acidic protein; TNFα, tumor necrosis factor α