Fig. 2

iPSC-derived CNSC-SE increased neuronal network activity and action potential bursts. a Overview of secretome treatment and MEA schedules. b MEA analysis of cortical neurons administered with CNSC-SE from day 50 to day 60. c MEA measurement on day 60. d Raster image of neuronal network activity over a period of 900 s. e Representative electrical activity of cortical neurons administered with CNSC-SE. As controls, iPSCs and cortical neurons were evaluated. Data are presented as mean ± SEM. One-way ANOVA; ^*#P < 0.05 (*iPSC vs. 130 μg/ml, #0 μg/ml vs. 130 μg/ml), Dunnett’s test. Kruskal–Wallis and Mann–Whitney analyses were performed for intergroup comparison; ^@P < 0.05. Abbreviations: MEA, multiwell microelectrode array; iPSC, induced pluripotent stem cell; CNSC-SE, cortical neural stem cell secretome