Fig. 7

PDDC treatment reduces tau spread in an AAV mutant hTau propagation model. a AAV-hTau model and dosing schematic. Mice were stereotaxically injected at 10 weeks old into the left dorsal hippocampus with AAV-CBA-hTau24(P301L)(S320F)-WPRE which was taken up and expressed by cells in the left CA3 and dentate gyrus and propagated to the right DG hilus region over the course of 6 weeks. b Representative images of the contralateral DG showing pThr181-Tau staining (green) from vehicle-treated (top) and PDDC-treated (bottom) AAV-hTau mice. Neurons stained with NeuN (magenta). Nuclei shown in blue. Scale bars, 50 µm. Gamma and brightness adjusted equally for all images presented. c Quantification of pThr181-Tau MFI of the contralateral DG normalized to the ipsilateral DG pThr181-Tau MFI. N = 81–84 images/group from 17 mice/group. d Quantification of the percentage of pThr181-Tau⁺ neurons in the contralateral dentate gyrus. N = 56–72 images/group from 17 mice/group. Unpaired two-tailed t-test. **P < 0.01. Bars represent mean ± SEM