Fig. 3

PDDC treatment reduces hippocampal tau levels in PS19 mice. a Representative Western blots from micro-dissected hippocampal tissue showing total human tau (upper blot) and pThr181-Tau (lower blot). GAPDH shown as a loading control. b Quantification of Western blots for total tau. c Quantification of Western blots for pThr181-Tau. d pThr181-Tau level normalized to total tau. N = 11–12/group. e–s Representative images showing pThr181-Tau staining (green) and neuronal staining (magenta) from vehicle- and PDDC-treated PS19 mice in the CA1 (e–h), CA3 (j–m) and dentate gyrus (DG, o–r). Single-cell mean fluorescence intensity (MFI) from the CA1 (i), CA3 (n), and DG (s). Nuclei shown in blue. N = 120 cells from 4 mice/group. Bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 20 µm. Gamma and brightness adjusted equally for all images presented. All graphs, unpaired two-tailed t-test