Fig. 6
From: α-Synuclein-carrying astrocytic extracellular vesicles in Parkinson pathogenesis and diagnosis
Development of a flow cytometry-based assay for astrocyte-derived EVs. a–c Representative histograms showing the populations of EVs positive for GLT-1, fluorophore-conjugated IgG isotype control, a blank (fluorophore only, no antibody) control experiment, and plasma with depletion of EVs positive for GLT-1 by ultracentrifugation. b Representative histograms showing the populations of EVs positive for SYN211, fluorophore-conjugated IgG isotype control, the blank control experiment, and plasma with depletion of EVs positive for SYN211 by ultracentrifugation. c Representative histograms showing the populations of EVs positive for MJFR14, fluorophore-conjugated IgG isotype control, the blank control experiment, and plasma with depletion of EVs positive for MJFR14 by ultracentrifugation. d–f Quantification data of positive EVs detected by the flow cytometry-based assay demonstrating the specificity of EV assays. g–i Linearity in different dilutions of EV plasma samples. j–l Stability of reference plasma (three replicates run each day on three separate days of the experiment for GLT-1, SYN211, and MJFR14)