Fig. 5
From: α-Synuclein-carrying astrocytic extracellular vesicles in Parkinson pathogenesis and diagnosis
Characterization and quantification of GLT-1+ EVs in human plasma. a Representative western blot images showing the levels of Alix, CD9, GFAP and GLT-1 in the EVs immune-enriched by the normal mouse IgG or the anti-GLT-1 antibody from human plasma, in human raw plasma and mouse brain homogenates. b A representative TEM image showing GLT-1 protein on the surface of EVs immune-enriched by the anti-GLT-1 antibody from human plasma. Scale bar, 100 nm. c The number of EVs immune-enriched from human plasma by the anti-GLT-1 antibody, the GLAST antibody, or the normal mouse IgG antibody. N = 3 independent repeats. d The general size and distribution of isolated GLT-1+ EVs analyzed by NTA. e MSD analysis of the level of α-syn in the EVs immune-enriched from human plasma by the GLT-1 antibody, the GLAST antibody, or the normal mouse IgG antibody. N = 3 independent repeats. f MSD analysis of the level of α-syn in the EVs immune-enriched by the GLT-1 antibody from normal human plasma, EV-poor plasma, and plasma pre-captured by GLAST. N = 3 independent repeats. Values are means ± S.E.M., one-way ANOVA followed by a Tukey’s post-hoc test. ***P < 0.005