Fig. 4

Increased autophagy in astrocytes with pathological α-syn stimulation. a Representative fluorescence images of LC3 (red) and nuclei (DAPI, blue) in astrocytes with overexpression of A53T α-syn. b Quantification of the LC3 particle number in astrocytes with overexpression of A53T α-syn. N = 12–16 astrocytes from 3 independent experimental repeats. c, d Western blots of LC3 in the astrocytes with overexpression of A53T α-syn and quantifications. N = 3 independent experimental repeats. e Representative fluorescence images of LC3 (red) and nuclei (DAPI, blue) in astrocytes treated with α-syn aggregates. f Quantification of the LC3 particle numbers in the astrocytes treated with α-syn aggregates. N = 12–16 astrocytes from 3 independent experimental repeats. g, h Western blots of LC3 in astrocytes treated with α-syn aggregates and quantifications. i, j Western blots of SQSTM1/p62 in the astrocytes with overexpression of A53T α-syn or treated with α-syn aggregates, and quantifications. N = 3 independent repeats. Values are means ± S.E.M., one-way ANOVA followed by a Tukey’s post-hoc test. *P < 0.05; **P < 0.01; ****P < 0.001