Fig. 3

The levels of lysosome-associated membrane proteins decreased in astrocytes with pathological α-syn stimulation. a–d Representative fluorescence images of Lamp1 (red), α-syn (green), and nuclei (DAPI, blue) in astrocytes with overexpression of A53T α-syn or α-syn aggregate exposure. Scale bars, 20 μm. e–h Representative fluorescence images of Lamp2 (red), α-syn (green) and nuclei (DAPI, blue) in astrocytes with overexpression of A53T α-syn or α-syn aggregate exposure. Scale bars, 3 μm. i, j Quantification of the relative fluorescent intensity of Lamp1 in astrocytes with overexpression of A53T α-syn (i) or aggregated α-syn exposure (j). N = 10 astrocytes from 3 independent repeats. k, l Quantification of the relative fluorescent intensity of Lamp2 in astrocytes with overexpression of A53T α-syn (k) or aggregated α-syn exposure (l). N = 10 astrocytes from 3 independent repeats. Values are means ± S.E.M., unpaired t-test. *P < 0.05; **P < 0.01; ****P < 0.001