Table 3 Primary and secondary antibodies used for immunofluorescence analysis
Antibodies used for iPSC characterization | ||||
|---|---|---|---|---|
Primary antibodies | Host species | Company | Catalogue number | Dilution |
Oct4 | Rabbit | Abcam (Human Embryonic Stem Cell Marker Panel) | ab109884 | 1:100 |
Tra-1–60 | Mouse | 1:100 | ||
Sox2 | Rabbit | 1:100 | ||
Nanog | Rabbit | 1:100 | ||
SSEA4 | Mouse | 1:100 | ||
Secondary antibodies | Host species | Company | Catalogue number | Dilution |
|---|---|---|---|---|
AlexaFluor 488 anti-rabbit | Donkey | Thermo Fisher Scientific | A-21206 | 1:2000 |
AlexaFluor 568 anti-mouse | Donkey | Thermo Fisher Scientific | A-10037 | 1:2000 |
Antibodies used for DA-iPSn characterization | ||||
|---|---|---|---|---|
Primary antibodies | Host species | Company | Catalogue number | Dilution |
TH | Rabbit | Millipore | AB5986 | 1:500 |
FOXA2 | Mouse | Santa Cruz | sc101060 | 1:400 |
TUBB3 | Mouse | Biolegend | 801202 | 1:1000 |
Secondary antibodies | Host species | Company | Catalogue number | Dilution |
|---|---|---|---|---|
AlexaFluor 488 anti-mouse | Donkey | Thermo Fisher Scientific | A-21202 | 1:500 |
AlexaFluor 594 anti-rabbit | Goat | Thermo Fisher Scientific | A-11037 | 1:500 |
Antibodies used for lysosomal protein stainings | ||||
|---|---|---|---|---|
Primary antibodies | Host species | Company | Catalogue number | Dilution |
CTSB | Goat | R&D systems | AF953 | 1:100 |
CTSD | Rabbit | Kindly provided by Prof. Andrej Hasilik, Philipps-University Marburg, Germany | N/A | 1:100 |
LAMP2 | Mouse | DSHB | H4B4 | 1:200 |
Secondary antibodies | Host species | Company | Catalogue number | Dilution |
|---|---|---|---|---|
AlexaFluor 488 anti-mouse | Donkey | Thermo Fisher Scientific | A-21202 | 1:500 |
AlexaFluor 596 anti-rabbit | Donkey | Thermo Fisher Scientific | A-21207 | 1:500 |
AlexaFluor 647 anti-goat | Donkey | Invitrogen | A-21447 | 1:500 |