Table 1 Primary and secondary antibodies used for Western blotting
Antibody | Host species | Company | Catalogue number | Dilution |
|---|---|---|---|---|
Primary antibody | ||||
αSyn (C-20) | Rabbit | Santa Cruz | sc-7011-R | 1:1000 |
αSyn (Poly) | Rabbit | Proteintech | 10842-1-AP | 1:1000 |
αSyn (Syn-1) | Mouse | BD | 610787 | 1:500 |
αSyn (LB509) | Mouse | Abcam | 27.766 | 1:500 |
αSyn (Syn-211) | Mouse | Santa Cruz | sc-12767 | 1:500 |
CTSB | Goat | R&D systems | AF953 | 1:500 |
CTSD | Rabbit | Kindly provided by Prof. Andrej Hasilik, Philipps-University Marburg, Germany | N/A | 1:500 |
CTSD | Mouse | BD | 610800 | 1:1000 |
CTSL | Goat | R&D systems | AF952 | 1:500 |
LAMP1 | Mouse | DSHB | H4A3 | 1:1000 |
LAMP2 | Rabbit | Novus Biologicals | NBP2-67298 | 1:1000 |
GAPDH | Rabbit | Cell signaling Technology | 14C10 | 1:1000 |
TUBB3 | Mouse | BioLegend | 802001 | 1:1000 |
β-actin | Mouse | Sigma | A5441 | 1:5000 |
Secondary antibody | ||||
Alexa Fluor 680 anti-mouse | Donkey | Thermo Fisher Scientific | A-100038 | 1:10,000 |
Alexa Fluor 680 anti-goat | Donkey | Thermo Fisher Scientific | A-21084 | 1:10,000 |
IRDye 800 anti-mouse | Donkey | Li-Cor | 926-32212 | 1:10,000 |
IRDye 680 anti-rabbit | Donkey | Li-Cor | 926-68073 | 1:10,000 |
IRDye 800 anti-rabbit | Donkey | Li-Cor | 926-32213 | 1:10,000 |
IRDye 800 anti-goat | Donkey | Li-Cor | 926-32214 | 1:10,000 |