Fig. 3

Treatment of FUS and Adu increased the number of activated phagocytic microglia associated with amyloid plaques in the hippocampus. a Representative images showing Iba-1-positive cells (green) surrounding Aβ plaques (red) in the dentate gyrus of the hippocampus. b Representative images of CD68 (red) and Iba-1(green) contained in the dentate gyrus of the hippocampus. c A bar graph showing the number of Iba1⁺microglia within 20 µm from Aβ plaques that were larger than 500 µm² or smaller than 500 µm². d A bar graph showing the total area of microglia. e A bar graph showing the phagosome area (CD68⁺/Iba1⁺) in microglia. Data are expressed as the means ± SEM. Statistical analyses were performed using one-way ANOVA, followed by Tukey’s post hoc analysis. (*P < 0.05 compared with 5×FAD-Sham mice, n = 3–4 mice for each group)