Fig. 5
From: Association of variants in the KIF1A gene with amyotrophic lateral sclerosis
Colocalization of VAMP2 with ALS-associated KIF1A mutants. a Cultured mouse cortical neurons (DIV7) were cotransfected with the FLAG-VAMP2 construct, along with the Venus-tagged WT and mutant KIF1A constructs. Neurons were fixed, immunostained with an anti-FLAG antibody, and imaged at DIV11. Scale bar, 5 μm. b Quantitative analysis of the percentage of VAMP2 colocalized with KIF1A in a. n = 19–28 neurons from three independent experiments. *P < 0.05; **P < 0.01; P = 0.0196 (WT vs R1356Q); P = 0.0030 (WT vs A1643V) by one-way ANOVA and Fisher’s test. Error bars represent SEM