Fig. 2

Biochemical abundance of pS129 αS in spinal cord is not affected by tau expression in end-stage TgA53T mice. a Representative immunoblots of total αS (a) and pS129 αS (b) in spinal cords of end-stage mice. c, d Quantitation of immunoblots shown in a and b. The level of pS129 αS was normalized to total αS. The levels of total αS and pS129 αS/total αS were not different between TgA53T and TgA53T/mTau^−/− mice in end-stage spinal cord lysates (t-test: P = 0.0518 and P = 0.4267, respectively). All quantified bands were normalized to the respective ponceau S total protein. Abbreviations: age-matched, A.M.; arbitrary units, A.U.; ponceau S, ponc; n = 5 animals/genotype; error bars represent mean ± SEM