Fig. 10

TrkB phosphorylation and activation are intact in Smn-deficient motoneurons. a Representative Western blot shows that pTrkB levels increase during 15 min BDNF stimulation in total cell lysates obtained from Smn+/+;SMN2tgtg but also Smn−/−;SMN2tgtg cultured motoneurons. b Representative images show pTrkB levels in the soma of cultured Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg motoneurons. c Graph shows mean intensities of pTrkB immunoreactivity in the soma. pTrkB levels increase significantly in the soma of Smn+/+;SMN2tgtg (**P = 0.0022; n = 54–61 cells) as well as Smn−/−;SMN2tgtg neurons (**P = 0.0019; n = 57–59 cells from 3 independent experiments) after 10 min BDNF pulse. Data are normalized to no BDNF group of Smn+/+;SMN2tgtg. Data are presented in scatter dot plot; error bars represent mean ± SEM. Statistical analyses were done by two-way ANOVA with Tukey multiple comparison post-test