Fig. 2

E46 and E83 arginylation targets the α-syn pool that sediments at lower centrifugation speeds in extracts from the frontal cortex, but does not strongly colocalize with Lewy bodies in the frontal cortex sections from human PD patients. a Magnification of a representative Western blot of the sample C1 probed with the total and modified α-syn antibodies, showing the doublet α-syn bands recognized by the total α-syn antibody and the relative position of the single band preferentially recognized by the antibodies to the modified α-syn variants as the upper band in this doublet. b Representative Western blots from the total brain extract, pellet, and supernatant fractions. Bands of heavily modified α-syn were preferentially found in the pellet, indicating its incorporation into the insoluble α-syn pool. c Representative images of human brain sections showing prominent LB formation, stained with antibodies to total α-syn, as well as the antibodies against E46- and E83-arginylated variants. LBs were prominently seen as large green dots in the top set of images stained with total α-syn antibodies. Most of these LBs show no specific staining with E83, and only occasional aggregates were visualized with E46 antibodies (short arrows). Both E46 and E83 antibodies also stained fibrillar aggregates (long arrows)