Fig. 1

E46- and E83-arginylated α-syn are present in the human frontal cortex and show varied levels in PD patients and healthy controls. a Representative immunoblots of total α-syn, as well as α-syn modified by phosphorylation at S129 or arginylation at E46 or E83. b Quantification of α-syn signal from all antibodies in all samples; for total α-syn, signals from both bands of the doublet in the ~ 14 kDa range were added, and the upper and lower bands of the doublet were also quantified separately. Error bars represent SEM from triplicate runs of the same samples. C, control; PD, Parkinson’s disease; PDD, Parkinson disease with dementia. P-value comparisons between control and PD (P12), control and PDD (P13), as well as PD and PDD (P23) are shown on top of each panel, calculated by two-tailed Welsh t-test. See Table S1 for further details on the patient samples