Fig. 1
From: Dense core vesicle markers in CSF and cortical tissues of patients with Alzheimer’s disease
Levels of secretory proteins in the AD cerebral cortex. Left Representative western blots showing protein levels of PC1/3, PC2, CPE, SgIII, CysC, Syp, and β-actin in homogenates of control and AD hippocampus. Each of the two immunoblotting columns illustrates levels of different proteins in the same cases blotted on the same membrane. The total membrane-transferred proteins were detected by Ponceau S staining. The mobility of molecular mass markers (in kDa) is indicated. Right Secretory (PC1/3, PC2, CPE, SgIII, CysC) and synaptic vesicle (Syp) protein levels in the parietal cortex (PCx) and hippocampus (HP) of AD patients compared to the age-matched controls (n = 7 for each tissue). Graphs show the percentage variation compared with controls and normalized to total membrane-blotted proteins. Levels of the precursor form of PC2 (proPC2) were increased in AD cortical tissues, whereas CysC dimers/aggregates were reduced in the PCx of AD patients. A consistent decrease of Syp is detected in both AD cortices, HP and PCx. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, Mann–Whitney test