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Table 1 Antibodies used in the study

From: Astrocytes and retrograde degeneration of nigrostriatal dopaminergic neurons in Parkinson’s disease: removing axonal debris

Antibody Dilution Host Producer, catalogue number
Primary antibody
APP 1:1500 Rabbit Abcam, ab32136
CD68 1:1000 Mouse AbD Serotec, MCA341GA
Cathepsin 1:100 Goat St John´s Lab, STJ140018
DAT 1:50 Goat Santa Cruz Biotechnology,Inc.,sc1433
GDNF 1:500 Rabbit Alomone, ANT-014
GFAP 1:1000 Mouse Millipore, MAB360
GFAP 1:1000 Rabbit Sigma, G9269
GFP (YFP) 1:1000 Rabbit Abcam, ab6556
IBA1 1:500 Rabbit Wako, 019-19741
IBA1 1:500 Mouse Millipore, MABN92
LAMP1 1:100 Mouse Abcam, ab25630
LAMP2 1:100 Goat Santa Cruz Biotechnology, Inc., sc8100
LC3-II 1:1200 Rabbit Abcam, ab48394
LC3-II 1:200 Goat Santa Cruz Biotechnology, Inc., sc16755
P62 (SQSTM1) 1:400 Guinea Pig Origene, BP5002
SYN 1:200 Rabbit Sigma, S3062; Millippore, AB5038
TH 1:1200 Chicken Abcam, ab76442
VMaT2 1:1500 Guinea Pig Origene, EUD2801
YFP 1:200 Goat St Johnsons lab, STJ140118
Secondary antibody
AF 488 anti-Chicken 1:400 Donkey Jackson ImmunoResearch, 703-545-155
AF 647 anti-Chicken 1:400 Donkey Jackson ImmunoResearch, 703-605-155
AF 488 anti-Goat 1:400 Donkey Jackson ImmunoResearch, 705-545-147
AF 647 anti-Mouse 1:400 Donkey Jackson ImmunoResearch, 715-605-151
Biotin anti-Mouse 1:300 Donkey Jackson ImmunoResearch, 715-065-151
Cy3 anti-Rabbit 1:400 Donkey Jackson ImmunoResearch, 711-165-152
Biotin anti-Rabbit 1:1000 Donkey Jackson ImmunoResearch, 711-065-152
AF 488 anti-Guinea Pig 1:1000 Goat Jackson ImmunoResearch, 106-545-003
Biotin anti-Guinea Pig 1:1000 Goat Jackson ImmunoResearch, 106-065-003
AF 488 anti-Chicken 1:800 Goat Invitrogen, A11039
AF 546 anti-Chicken 1:600 Goat Invitrogen, A11040
AF 546 anti-Mouse 1:1000 Goat Invitrogen, A21123
AF 488 anti-Mouse 1:400 Goat Jackson ImmunoResearch, 115-546-146
Biotin anti-Mouse 1:1200 Goat Jackson ImmunoResearch, 115-065-003
Cy3 anti-Rabbit 1:500 Goat Jackson ImmunoResearch, 111-165-144
Biotin anti-rabbit 1:1200 Goat Jackson ImmunoResearch, 111-065-144
Others
405-streptavidin 1:400 Jackson ImmunoResearch, 016-470-084
Dylight 649-streptavidin 1:600 Jackson ImmunoResearch, 016-490-084
488-streptavidin 1:800 Jackson ImmunoResearch, 016-540-084
Cy3-streptavidin 1:600 - Jackson ImmunoResearch, 016–160-084
  1. TH: tyrosine hydroxylase, DAT: dopamine transporter, GFAP: glial fibrillary acidic protein, App: amyloid precursor protein, Syn: α-synuclein, GDNF: glial-derived neurotrophic factor, Lamp1: lysosomal-associated membrane protein 1, Lamp2: lysosomal-associated membrane protein 2, CD68: cluster of differentiation 68, LC3: microtubule-associated protein 1A/1B-light chain 3, VMat2: vesicular monoamine transporter 2, Iba1: ionized calcium binding adaptor molecule 1, YFP: yellow fluorescent protein