Table 1 Antibodies used in the study
Antibody | Dilution | Host | Producer, catalogue number |
|---|---|---|---|
Primary antibody | |||
APP | 1:1500 | Rabbit | Abcam, ab32136 |
CD68 | 1:1000 | Mouse | AbD Serotec, MCA341GA |
Cathepsin | 1:100 | Goat | St John´s Lab, STJ140018 |
DAT | 1:50 | Goat | Santa Cruz Biotechnology,Inc.,sc1433 |
GDNF | 1:500 | Rabbit | Alomone, ANT-014 |
GFAP | 1:1000 | Mouse | Millipore, MAB360 |
GFAP | 1:1000 | Rabbit | Sigma, G9269 |
GFP (YFP) | 1:1000 | Rabbit | Abcam, ab6556 |
IBA1 | 1:500 | Rabbit | Wako, 019-19741 |
IBA1 | 1:500 | Mouse | Millipore, MABN92 |
LAMP1 | 1:100 | Mouse | Abcam, ab25630 |
LAMP2 | 1:100 | Goat | Santa Cruz Biotechnology, Inc., sc8100 |
LC3-II | 1:1200 | Rabbit | Abcam, ab48394 |
LC3-II | 1:200 | Goat | Santa Cruz Biotechnology, Inc., sc16755 |
P62 (SQSTM1) | 1:400 | Guinea Pig | Origene, BP5002 |
SYN | 1:200 | Rabbit | Sigma, S3062; Millippore, AB5038 |
TH | 1:1200 | Chicken | Abcam, ab76442 |
VMaT2 | 1:1500 | Guinea Pig | Origene, EUD2801 |
YFP | 1:200 | Goat | St Johnsons lab, STJ140118 |
Secondary antibody | |||
AF 488 anti-Chicken | 1:400 | Donkey | Jackson ImmunoResearch, 703-545-155 |
AF 647 anti-Chicken | 1:400 | Donkey | Jackson ImmunoResearch, 703-605-155 |
AF 488 anti-Goat | 1:400 | Donkey | Jackson ImmunoResearch, 705-545-147 |
AF 647 anti-Mouse | 1:400 | Donkey | Jackson ImmunoResearch, 715-605-151 |
Biotin anti-Mouse | 1:300 | Donkey | Jackson ImmunoResearch, 715-065-151 |
Cy3 anti-Rabbit | 1:400 | Donkey | Jackson ImmunoResearch, 711-165-152 |
Biotin anti-Rabbit | 1:1000 | Donkey | Jackson ImmunoResearch, 711-065-152 |
AF 488 anti-Guinea Pig | 1:1000 | Goat | Jackson ImmunoResearch, 106-545-003 |
Biotin anti-Guinea Pig | 1:1000 | Goat | Jackson ImmunoResearch, 106-065-003 |
AF 488 anti-Chicken | 1:800 | Goat | Invitrogen, A11039 |
AF 546 anti-Chicken | 1:600 | Goat | Invitrogen, A11040 |
AF 546 anti-Mouse | 1:1000 | Goat | Invitrogen, A21123 |
AF 488 anti-Mouse | 1:400 | Goat | Jackson ImmunoResearch, 115-546-146 |
Biotin anti-Mouse | 1:1200 | Goat | Jackson ImmunoResearch, 115-065-003 |
Cy3 anti-Rabbit | 1:500 | Goat | Jackson ImmunoResearch, 111-165-144 |
Biotin anti-rabbit | 1:1200 | Goat | Jackson ImmunoResearch, 111-065-144 |
Others | |||
405-streptavidin | 1:400 | – | Jackson ImmunoResearch, 016-470-084 |
Dylight 649-streptavidin | 1:600 | – | Jackson ImmunoResearch, 016-490-084 |
488-streptavidin | 1:800 | – | Jackson ImmunoResearch, 016-540-084 |
Cy3-streptavidin | 1:600 | - | Jackson ImmunoResearch, 016–160-084 |