Fig. 8

Inhibition ELISA illustrating the different binding strengths of Hexa-RmAb158 to Aβ. Five different species of Aβ used in this setup: insoluble fibrils, protofibrils in fraction 1, oligomers in fraction 3, oligomers in fraction 4, and Aβ40 monomers. a Schematic representation of inhibition Aβ ELISA, where a pre-incubated Aβ/antibody mixture is added to a protofibril-coated plate. b Hexa-RmAb158 displaying a highly selective binding to the protofibrils and both the oligomeric fractions, with moderate binding strength to the fibrils and weak binding to the monomers. The concentrations were log transformed, and the obtained OD values were normalized to 100% binding, where the highest OD value is defined as 100% binding, and OD value of zero is defined as 0% binding. Data are presented as mean ± SD, n = 2–3