Fig. 1

Acrolein and α-syn expression tested by immunofluorescence and Western blot in 6-OHDA rats with DP treatment. a Acrolein immunoreactivity was significantly increased in both substantia nigra (SN) and striatum of the 6-OHDA-injected group compared to the sham group when examined 5 weeks post injection. However, this 6-OHDA-mediated increase in acrolein immunoreactivity was alleviated by daily intraperitoneal injection of DP. b, c Quantitative analysis of acrolein immunoreactivity in a. n = 5 in each group. d Western blots of acrolein and α-syn in the SN. e Quantitative analysis of band intensity in d. Statistical analysis showed significant increases of both acrolein and α-syn after 6-OHDA toxicity, which were alleviated by DP injection. *P < 0.05, **P < 0.01, ANOVA. Data are expressed as mean ± SD. Scale bar, 1 mm