Fig. 2

α-Synuclein particles and acidic compartments by light microscopy. a Deconvolved confocal images of live HEK293T cells transfected with A53T-α-synuclein (Syn)-EGFP and treated with lysotracker red for 2 h at 37 °C. Arrows show an aggresome. Note the distribution of lysotracker-positive vesicles close to the aggresome and other αSyn aggregates. b Deconvolved confocal image of HEK293T cells transfected with A53T-α-Syn-mCherry, the lysosomal marker LAMP1-EGFP and the cargo protein p62 without fluorescent tag. Enlarged insets show a cluster of LAMP1-decorated vesicles colocalizing with aSyn. Arrow shows a large aggresome, so called “p62 body”. c Deconvolved confocal image of HEK293T cells transfected with A53T-α-syn-EGFP and a biosensor of the autophagosome lipid phosphatidylinositol 3-phosphate (PI3P) tagged to mCherry. Enlarged insets show the PI3P-positive vesicles around the aggresome (arrow), consistent with the hypothesis that autophagosomes degrade aggresomes. In panels b-c, the red and green channels of the insets are shown individually next to the merged images. Scale bars, 5 μm. Original data