Fig. 3

HUMSCs transplantation alleviated molecular layer atrophy in six-month-old SCA1 mice. Six-month-old mice were sacrificed to examine alterations within cerebellar sagittal sections (a1). The areas of cerebellar sagittal section were quantified. HUMSCs transplantation reduced the shrinkage of cerebellum in SCA1 mice (b). All groups were examined, photomicrographs of entire sagittal sections were stained with Cresyl violet (a2-a6), lobule III at low (red arrow, a2 and a3) and high (a4) magnification, and lobule VI at low (blue arrow, a2 and a5) and high (a6) magnification were shown. In lobules III and VI, significant reductions were found in the molecular layer areas (c and d) and thickness (f and g) in the six-month-old SCA1 and SCA1-PBS groups. HUMSCs transplants could partially mitigate these reductions in SCA1-HUMSCs mice. There were no significant differences in the body weight among mice in all groups at the same ages (e). * p < 0.05 compared with the Normal group. ▲p < 0.05 compared with the Normal-PBS group. # p < 0.05 compared with the SCA1-HUMSCs group