Fig. 1

HUMSCs transplantation ameliorated motor behavior deterioration in SCA1 mice. a Scheme of experimental procedures. b Genotyping of SCA1 transgene. SCA1 Primer 1 set amplified a 500-bp amplicon. Lanes 3 and 4 show the presence of the SCA1 transgene. c The sketch and HE-stained picture show the injection site of HUMSCs. Open field tests revealed that HUMSCs transplants improved total distance traveled, rearing frequency, and rearing time in the SCA1-HUMSCs group mice (d and e). The group of SCA1-HUMSCs received, at 1 month of age, the HUMSCs transplantation which ameliorated the significant shortening of the latency to fall in the rotarod test (f), flat-forward, descending, and ascending speeds in the balance beam test (g) suffered by the SCA1 mice. * p < 0.05 compared with the Normal group of the same age. ▲ p < 0.05 compared with the Normal-PBS group of the same age. # p < 0.05 compared with the SCA1-HUMSCs group of the same age