Fig. 4

Drug effects on α-syn aggregates and co-localization of LAMP-1, LAMP-2A in the SynT-aggregation model. H4 cells were co-transfected with SynT and synphilin-1. (a-b) Cells were fixed 48 h post-transfection and subjected to IF for α-syn (red) and LAMP-1/-2A (green) followed by confocal microscopic analysis. (c) LAMP-1 and LAMP-2A showed an increased expression level in SynT transfected H4 cells following treatment with DHM (10 μM, 48h) and Sal B (50 μM, 48h), compared to untreated cells. The fluorescence density levels of α-syn were decreased in DHM and Sal B treated cells compared with the control. (d) The co-localization levels of LAMP-1/-2A with α-syn in intracellular aggregates were increased in DHM and Sal B treated cells. All data shown are representative of at least three independent experiments (mean ± SD, *p<0.05, **p<0.01), Scale bar = 5 μm