Fig. 3

Expression of LAMP-1 and LAMP-2A in α-syn transfected H4 cells. A control group without any transfection was performed as a ‘mock’ while another group with an ordinary wildtype α-syn plasmid transfection was performed as ‘WT α-syn’. SynT and synphilin 1 co-transfected group was performed as the experimental group with DHM, Sal B, Rapamycin or Chloroquine treatments. The expression levels of LAMP-1/-2A, SynT or endogenous α-syn were detected by Western blot (a). Quantitative analyses of α-syn (SynT) and LAMP-1/-2A normalized to β-actin (B-E) (n = 5). Expression of LAMP-1 (b) and LAMP-2A (c) was significantly increased after treatment with DHM or Sal B, as compared with WT α-syn transfected cells, rapamycin treated cells or CQ treated cells (after normalization to untreated cells). (d) SynT levels were decreased in both DHM and Sal B treated cells as compared to the SynT-aggregation model or (CQ) treated cells. (e) WT α-syn expression levels were increased in DHM (10 μM) or Sal B (50 μM) treated cells as compared with the SynT-aggregation model. All data shown are representative of at least three independent experiments (mean ± SD, *p<0.05, **p<0.01)