Erythrocytic α-Synuclein as a potential biomarker for Parkinson’s disease

Table 2 Erythrocytic levels of total, phosphorylated, and aggregated α-Syn in patients with PD and healthy controls

Analyte	Fraction	Raw (±SEM) pg/ml			Normalized(±SEM) pg/μg
		PD	Control	P	PD	Control	P
Total α-Syn^a	Cytosol	33,942,290.92 ± 56,783.75	34,160,888.80 ± 102,781.46	0.45	76.44 ± 0.08	85.34 ± 0.26	0.203
Total α-Syn^a	Membrane	3,443,923.88 ± 8521.66	3,169,731.45 ± 15,787.25	0.005	108.60 ± 0.26	94.71 ± 0.26	0.008
Aggregated α-Syn^b	Cytosol	126,184.16 ± 210.16	131,977.36 ± 502.61	0.10	4024.52 ± 7.86	4316.47 ± 20.66	0.47
Aggregated α-Syn^b	Membrane	85,028.97 ± 184.99	56,531.94 ± 190.85	< 0.0005	27,284.05 ± 44.17	21,098.82 ± 75.82	<0.0005
PS129^b	Cytosol	18,223.73 ± 155.50	2028.41 ± 13.10	< 0.0005	636.05 ± 6.03	67.36 ± 0.48	<0.0005
PS129^b	Membrane	891.03 ± 2.23	584.16 ± 3.87	< 0.0005	315.35 ± 0.95	255.05 ± 1.98	<0.0005

^a Total α-Syn concentrations were normalized to the total protein levels in the same erythrocytic subcellular compartment, and are expressed in unit of pg (total α-Syn)/μg (total protein)
^b Oligomeric or phospho (pS129) α-Syn concentrations were normalized to the corresponding total α-Syn levels, and are expressed in units of pg (oligo α-Syn)/μg (total α-Syn) and pg (pS129)/μg (total α-Syn), respectively
PD Parkinson’s disease, SEM standard error of the mean, α-Syn α-synuclein

ISSN: 2047-9158