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Fig. 5 | Translational Neurodegeneration

Fig. 5

From: Constipation, deficit in colon contractions and alpha-synuclein inclusions within the colon precede motor abnormalities and neurodegeneration in the central nervous system in a mouse model of alpha-synucleinopathy

Fig. 5

αS soluble and insoluble HMW species in the colon increase with age in presymptomatic mice. Time course analysis of soluble and insoluble fractions obtained from the proximal (P) and distal (D) colon of presymptomatic Tg mice at different age shows an increase in the accumulation of αS HMW species. NP-40 soluble (a) and insoluble (d) lysates of proximal and distal colon were isolated from presymptomatic Tg mice and nTg littermates at different age, run on a SDS-Page and blotted with Syn-1, pSer129-αS and GAPDH antibodies. Soluble and insoluble phospho-HMW αS species were already abundantly present in mice at 3 months of age and their level exacerbated with age, reaching a plateau at 6 months. The distal colon appears to be the intestinal region with the highest presence of αS oligomers and aggregates. b, c, e, f Graphs showing relative density of HMW αS (b, e) or phospho-αS (c, f) in all the fractions analyzed. Values on graphs are expressed as % relative to nTg and are given as the mean ± SEM (n = 3–4 per group). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, # p < 0.05; ## p < 0.01; ### p < 0.001; #### p < 0.0001, where * refers to comparisons between Tgs and their nTg age-matched controls, while # refers to comparisons between distal and proximal colon of Tgs; two-way ANOVA followed by Fischer’s LSD test

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Translational Neurodegeneration

ISSN: 2047-9158

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