Fig. 5
From: Suppression of astrocytic autophagy by αB-crystallin contributes to α-synuclein inclusion formation
Knockdown of CRYAB in astrocytes promotes α-synuclein degradation. a A schematic shows the timeline of treatment of U251 cells. Cells were transfected with siRNA of CRYAB were exposed to 0.4 μM α-synuclein PFFs for 24 h, washed thoroughly, and cultured for an additional 0 and 3 days in α-synuclein-free medium with 10 μM CQ stimulation before fixation. b-c The intracellular location of the α-synuclein after ingestion in day 0 and day 3 without CQ stimulation was confirmed with confocal imaging. Scale bars: 1 μm. The graph shows a statistical analysis of the mean intensity of the intracellular α-synuclein per field in cells. Data are presented as mean ± SEM, n = 40. d The intracellular location of the α-synuclein after ingestion in day 0 and day 3 without CQ stimulation was confirmed with confocal imaging. The graph shows a statistical analysis of the mean intensity of the total area of the α-synuclein deposits per cell. Data are presented as mean ± SEM, n = 40. e The graph shows a statistical analysis of the mean intensity of the number of α-synuclein deposits per cell. Data are presented as mean ± SEM, n = 40. f, g The intracellular location of the α-synuclein after ingestion on day 3 with CQ stimulation was confirmed with confocal imaging. Scale bars: 1 μm. The graph shows a statistical analysis of the mean intensity of the intracellular α-synuclein per field in cells. Data are presented as mean ± SEM, n = 40. h Western blotting analysis shows that knockdown CRYAB does not affect BAG3 expression levels. Data are expressed as mean ± SEM, n = 3. n.s. no significance. *P > 0.05