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Fig. 4 | Translational Neurodegeneration

Fig. 4

From: Suppression of astrocytic autophagy by αB-crystallin contributes to α-synuclein inclusion formation

Fig. 4

CRYAB inhibits the BAG3-dependent autophagy in U251 cells. a U251 cells expressing CRYAB stable were transfected with plasmids encoding BAG3-Myc-2A-GFP. The cultures were fixed 48 h after transfection and immunostained with GFP and LC3 antibodies. Scale bars: 10 μm. b Quantification of the number of LC3+ dots per cell shown in (A). Data are presented as mean ± SEM, n = 30 cells. c, d Representative immunoblot shows that BAG3 overexpression elevates the conversion of LC3-II to LC3-I in U251 cells. Cells were transfected with BAG3 plasmids for 48 h. Data are presented as mean ± SEM, n = 5. Unpaired t-test. **P < 0.01

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