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Fig. 3 | Translational Neurodegeneration

Fig. 3

From: Suppression of astrocytic autophagy by αB-crystallin contributes to α-synuclein inclusion formation

Fig. 3

Overexpression of CRYAB inhibits binding between BAG3 and HSC70 in vitro. a Co-immunoprecipitation (co-IP) reveals that CRYAB interacts with BAG3 in U251 cells. Data are from three independent experiments. b Double immunofluorescence staining. Some CRYAB+ (green) cells (arrowheads indicate examples) express BAG3 (red) in the U251 cell cultures. Right panel is an enlarged view of the box in the left panel. Scale bars: 10 μm. c Co-IP analysis reveals that BAG3 binds to HSC70 and HSPB8. d Co-IP analysis reveals that CRYAB overexpression inhibits binding between BAG3 and HSC70 in U251 cells. Data are from three independent experiments

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