Fig. 8
From: Intranasal rapamycin ameliorates Alzheimer-like cognitive decline in a mouse model of Down syndrome
InRapa reduces protein oxidative damage and K63 poly-ubiquitinylation. In panel a are shown the total hippocampal protein-bound 3-NT and HNE of Ts65Dn mice and Eu treated with Veh and InRapa analyzed by slot blot assay. In panel b are shown the hippocampal total poly-ubiquitin, poly-ubiquitin Lys63 and poly-ubiquitin Lys48 levels analyzed by Western blot. In panel c the quantification of panel b is reported. Densitometry values of each lane is the result of the sum of all the bands, analyzed by Image lab software as previously reported [10]. Graph values are the mean of 8 samples per each group, normalized per total load and given as percentage of Eu Veh, set as 100%