Fig. 6

Forced expression of Foxa2/Lmx1a in AiNPCs enhances dopaminergic neuron generation. a, b Following overexpression of Foxa2/Lmx1a, the levels of Foxa2 and Lmx1a in AiNPCs were determined by qPCR. c-j Augmentation of TH⁺/Nurr1⁺ dopaminergic neuron generation after transduction of Foxa2 (d, h), Lmx1a (e, i), and Foxa2/Lmx1a (f, j) in AiNPCs were determined by immunocytochemistry. k, l The percentage of TH⁺/Nurr1⁺ neurons in panels c-j was quantified by counting TH⁺/Nurr1⁺ cells and comparing against the total number of Tuj⁺ neurons. *P < 0.05, **P < 0.01 by two-tailed t test (n = 3). m Neuronal subtype marker gene expressions in Foxa2- and Lmx1a-transduced AiNPCs under mesencephalic cues were determined by qPCR. Scale bars represent 10 μm (c-j). Error bars denote s.d. from triplicate measurements (a, b, k-m)