Fig. 4

β5i inhibition exacerbates 6-hydroxydopamine-induced DA neuronal damage. a Cytotoxic effect on the SN4741 cell line of different treatments for 24 h analyzed by CCK8 and DCFH-DA tests (n = 5 experiments). b Immunofluorescence staining of β5i in SN4741 cells. Scale bar = 100 μm. c, d The expression of Caspase-3 and cleaved Caspase-3 in SN4741 cells after treatment with 6-OHDA or PR-957 for 24 h. e, f Immunohistochemistry of TH⁺ cells in rats after 6-OHDA treatment for 4 weeks. Scale bar = 200 μm. g Apomorphine-induced rotation scores after 6-OHDA treatment for 4 weeks (n = 6 experiments). Data are presented as the mean ± SD; n = 4 experiments; * P < 0.05, compared with control; ^# P < 0.05, compared with the 6-OHDA group; one-way ANOVA