Fig. 4
From: Identification of Ser465 as a novel PINK1 autophosphorylation site
S465 has no influence on PINK1 subcellular localization. a, b, c, the subcellular localization in HEK 293 cells transfected with pKH3, as negative control; (d, e, f), the subcellular localization in HEK 293 cells transfected with pKH3-PINK1-WT; (g, h, i), the subcellular localization of pKH3-PINK1-S465A transfected cells; (j, k, l), the subcellular localization of pKH3-PINK1-S465D transfected cells. In A, D, G and J, the red fluorescence illustrated the subcellular localization of proteins containing the HA tag; In B, E, H and K, the green fluorescence stood for mitochondria; In C, F, I and L, co-localization (in yellow) of both HA-tag containing protein (in red) and mitochondria (in green)