Fig. 2
From: Identification of Ser465 as a novel PINK1 autophosphorylation site
PINK1 protein autophosphorylation can be weakened by silencing S465 phosphorylation site. a Coomassie blue staining of purified GST-PINK1-S465A fusion protein. b Coomassie blue staining of three kinase phosphorylation reaction systems (GST, GST-PINK1-WT and GST-PINK1-S465A). GST protein band was shown in lane 1, as a negative control. Visible GST-PINK1-WT and GST-PINK1-S465A fusion protein bands were shown in Lane 2 and lane 3 respectively, as shown by asterisk. c Autoradiography results of three groups. Two positive bands were shown in lane 2 and 3, which stands for the autophosphorylation level of the two groups with GST-PINK1-WT and GST-PINK1-S465A as the kinases, respectively. No positive bands in lane 1. d Quantification of the autophosphorylation activity of GST-PINK1-WT and GST-PINK1-S465A protein based on the result of autoradiography. Compared to the WT fusion protein, the autophosphorylation level of mutant GST-PINK1-S465A protein was lower. *P < 0.05, statistically significant