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Table 5 Kinetics of the enzyme activity in full-length GAC, KGA, and their C-terminal deletion mutants

From: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration

Protein Km (mM) Vmax (μmol/min per ml)
GAC FL 28.62 ± 5.37 45.37 ± 4.08
KGA FL 21.69 ± 3.55 35.44 ± 2.48
GLS1 1–150 AA 0.63 ± 0.32 1.30 ± 0.05
GLS1 1 - 247AA 0.69 ± .039 0.92 ± 0.05
GLS1 1–344 AA 0.44 ± 0.26 0.84 ± 0.04
GLS1 1–450 AA 0.66 ± 0.39 0.76 ± 0.03
GLS1 1–550 AA 28.13 ± 3.30 46.14 ± 2.60