Fig. 2

HFD/STZ treatment casused cognitive impairment and depressive symptoms in mice. a and b, typical swimming traces of control mice (a) and diabetic mice (b) in Morris watermaze test. c, No difference were found between the two groups in the latency time during training trials. d, During testing phase, the frequency of platform-crossing in diabetic mice decreased remarkably when compared with control mice. (*P < 0.05, n = 12 for each group). Gand F, cued and contextual fear memory was evaluated using the fear conditioning test. Percent of time in total freezing during the contextual test (e) and tone test (f) was represented. (*P < 0.05, n = 12 for each group). Gand H, effects of diabetes on immobility time in the tail suspension test (g) and the forced swimming test (h). The immobility time in diabetes group was significantly higher than that of control group. (*P < 0.05, n = 12 for each group)