Fig. 3

α-syn is unable to undergo cell–to–cell transmission at SNc. a Sample images show the expression pattern of hα-syn (green) and TH (red) in the sagittal sections of 1–month–old Pitx3–tTA::tetO–SNCA bigenic mice. Topro3 was used for counter–staining (blue). Scale bar: 1 mm. b Sample images show the staining of hα-syn (green) and TH (red) in the SNc of 1– and 16–month–old Pitx3–tTA::tetO–SNCA bigenic mice. Scale bar: 100 μm. c Images highlight the boxed areas in b. Arrowheads point to the hα-syn–negative DA neurons. d Scatter plot depicts co-localization percentages at 1 month and 16 months. Data were presented as mean ± SEM. *P < 0.05