α-syn uptake in human primary astrocytes. (i) Confocal microscopy analysis of α-syn uptake after 30 and 60 min in human astrocytes. Staining for Alexa-Fluor-488-labelled α-syn in human neurons: Top, double staining for GFAP/red and DAPI/blue; Centre, double staining for α-syn/green and DAPI/blue; Bottom, Merged GFAP/red, α-syn/green and DAPI/blue. (ii) The histogram represents the semi-quantification of the immunostaining intensity. Intensity has been quantified using ImageJ 10.2. This experiment has been performed in triplicate with primary cultures of astrocytes prepared from three different brain samples. Three individual microscopic fields were analysed for each treatment and the SEM for the data was determined to be 5%. * indicate the level of significance (p < 0.05).