Fig. 2
From: α-Synuclein conformers reveal link to clinical heterogeneity of α-synucleinopathies
Immunohistochemical detection of α-synuclein (αSyn) deposits in different types of α-synucleinopathies. Original immunohistochemical staining from 2-µm sections of the cerebral cortex, cerebral white matter, and the midbrain including the substantia nigra was done on a Ventana Benchmark XT Autostainer (Ventana, Tucson, AZ), in accordance with the manufacturer's recommendations, using antibodies against αSyn (polyclonal; Zytomed 519–2684; dilution 1:1000). a Negative control. A person with no clinical history of Parkinson’s disease (PD), PD-associated dementia or dementia with Lewy bodies (DLB) showed physiological neuromelanin-containing neurons in the substantia nigra but no αSyn-positive structures. b A patient with a history of PD showed numerous αSyn-positive Lewy bodies and neurites in the substantia nigra. c αSyn-positive Lewy neurites in the neocortex of a patient with DLB are shown. d Numerous αSyn-positive glial cytoplasmic inclusions are recognizable in the white matter of a patient with multiple system atrophy