Fig. 5

Treatment with FUS and Adu increased neurogenesis in the hippocampus. a Representative images showing immunofluorescence of neuronal nuclear marker (NeuN, green) and 5-bromo-2′-deoxyuridine (BrdU, red) in the dentate gyrus of the hippocampus. Scale bars: 100 µm. SGZ: subgranular zone, GCL; granular cell layer. b Bar graphs showing the number of BrdU- and BrdU/NeuN-positive cells after the 1st treatment. c Bar graphs showing the number of BrdU- and BrdU/NeuN-positive cells after the 3rd treatment. Data are expressed as the means ± SEM. Statistical analyses were performed using one-way ANOVA followed by a least significant difference post-hoc analysis. (*P < 0.05, and **P < 0.01, compared with WT-Sham mice, ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 compared with 5×FAD-Sham mice, ^$P < 0.05 compared with 5×FAD + Adu mice, ^@P < 0.05 compared with 5×FAD + FUS mice, n = 5–10 for each group)