Skip to main content
Fig. 1 | Translational Neurodegeneration

Fig. 1

From: ImmunoPET imaging of amyloid-beta in a rat model of Alzheimer’s disease with a bispecific, brain-penetrating fusion protein

Fig. 1

Generation of bispecific fusion proteins with OX26 affinity variants. a Biacore analysis of affinity to rTfR for OX265, OX2676 and commercial OX26LSBio. b Schematic of the IEDDA reaction to generate OX26-F(ab′)2-Bapi bispecific fusion proteins. c, d SDS-PAGE analysis of OX265-F(ab′)2-Bapi (c) and OX2676-F(ab′)2-Bapi (d). Lanes 1, molecular weight ladder; 2, parental OX26 IgG; 3, F(ab′)2-Bapi; 4–5, preparative SEC fractions for the final pool of bispecific fusion protein. e, f ELISA analysis of OX265-F(ab′)2-Bapi and OX2676-F(ab′)2-Bapi before and after 125I-labelling binding to rTfR (e) and Aβ (f). RU, response unit; OD, optical density

Back to article page